MELANOMA-IMPFSTOFF UND SEINE HERSTELLUNG UND VERWENDUNG

摘要

An immunotherapeutic vaccine providing antigen presenting cells that have be en pulsed with a disrupted cell preparation which includes enucleated cytosol a nd cell membranes of cancer cells infected with a recombinant vaccinia virus encoding at least one immunostimulating molecule. In a preferred embodiment, the vaccine includes autologous dendritic/monocytic cells (DC/M) that presen t a mixture of antigens (present in the enucleated cytosol and cell membranes) from melanoma cell lines that have been infected with a recombinant vaccinia virus encoding IL-2. In another of the preferred embodiments, the enucleated cytosol and cell membranes are from melanoma cells harvested from the patien t to be treated. A method of making the vaccine and methods of using the vacci ne to stimulate an anti-cancer immune response and to treat a patient with a cancer are also described.