METHOD FOR CHROMATOGRAPHY

摘要

The present invention relates to a method for chromatography. More closely, the method relates to a novel way of separating proteins by chromatography with results that previously only have been achieved by electrophoresis. The invention relates to a method for chromatography of proteins, comprising the following steps: a) solubilising a protein sample in a solubilising agent; b) adsorbing solubilised proteins on a chromatographic column; c) eluting proteins from the column with a gradient of surfactant solvent; and d) collecting fractions comprising eluted proteins. The invention also relates to a kit and fraction collector for use in the method.