发明名称 |
FLAVIN ADENINE DINUCLEOTIDE-BINDING GLUCOSE DEHYDROGENASE |
摘要 |
<p>The object is to provide a novel enzyme which enables to determine the glucose level more accurately, a bacterium capable of producing the enzyme, and use of the enzyme. Disclosed is a flavin adenine dinucleotide-binding glucose dehydrogenase having the following properties (1) to (3): (1) the enzyme has an activity of catalyzing a reaction for oxidizing a hydroxyl group in glucose in the presence of an electron acceptor to produce glucono-d-lactone; (2) the enzyme has a molecular weight of about 100 kDa as measured by SDS-polyacrylamide gel electrophoresis or about 400 kDa as measured by gel filtration chromatography; and (3) the enzyme is less reactive to maltose, D-fructose, D-mannose and D-galactose. Also disclosed is a microorganism Aspergillus oryzae which can produce the enzyme. Further disclosed are a glucose determination method, a reagent for the determination of glucose, and a kit for the determination of glucose, each utilizing the enzyme.</p> |
申请公布号 |
WO2007139013(A1) |
申请公布日期 |
2007.12.06 |
申请号 |
WO2007JP60694 |
申请日期 |
2007.05.25 |
申请人 |
TANAKA, NORIAKI;AMANO ENZYME INC.;YUUKI, KENSUKE |
发明人 |
TANAKA, NORIAKI;YUUKI, KENSUKE |
分类号 |
C12N9/04;C12N1/14;C12Q1/32;C12R1/69;G01N21/75 |
主分类号 |
C12N9/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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