| 摘要 |
<p>It is intended to provide an immunoassay method which is appropriately conducted on a chip. Namely, an immunoassay method comprising: forming an antigen-antibody complex, wherein an antigen (2) to be assayed is bonded to an antibody (3), in a reaction chamber (7); and measuring the amount of the antigen-antibody complex or the antibody not binding to the antigen to be assayed by using an assay solution prepared by using a sample solution (8) as a solvent. According to this method, the assay solution for detecting a signal indicating the amount of the antigen to be assayed can be obtained without resorting to a buffer solution free from no substance to be assayed such as a protein. Therefore, it is unnecessary to supply the buffer from the outside of the chip or preliminarily allow the chip to hold the buffer, which makes it possible to easily conduct the immunoassay on the chip.</p> |
