METHODS AND SYSTEMS FOR INCREASING PRODUCTION OF EQUILIBRIUMREACTIONS

摘要

Methods and systems for increasing the production of equilibrium reaction s are described. In some embodiments, a method comprises removing enzymes fr om a reaction mixture once equilibrium is achieved, selectively re-adding en zymes and reactants for driving the product-forming reaction to product, and optionally recycling stabilized intermediates, by-products and/or co-produc ts back into the reaction mixture. In some embodiments, a method comprises p urifying the desired product from a reaction mixture and selectively recycli ng one or more components of the reaction mixture, such as original reactant s, intermediates, co-products or by-products back into the reaction mixture as desired to improve titer. Systems for implementing the methods are also p rovided. In some embodiments the methods and systems are implemented for inc reasing the production of monatin and monatin derivatives, produced in multi -step equilibrium pathways.