| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for preparation of a recombinant influenza vaccine using DNA technology. SOLUTION: The resulting vaccine is a multivalent, preferably trivalent, influenza vaccine based on a mixture of recombinant hemagglutinin antigens cloned from influenza viruses having epidemic potential. The recombinant hemagglutinin antigens are full length, nondecomposed (HA0), produced from baculovirus expression vectors in cultured insect cells and purified under non-denaturing conditions. In a preferred embodiment, the cloned HA genes are then modified by deletion of the natural hydrophobic signal peptide sequences and replacing them with a new baculovirus chitinase signal peptide. COPYRIGHT: (C)2008,JPO&INPIT
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