| 摘要 |
It is an object to provide a method of suitably analyzing the amount of gene expression of a single-cell. A method of detecting a nucleic acid comprising
a step of sampling a single-cell from a sample containing at least a single-cell,
a cell lysis step of lysing cell membrane of the sampled single-cell and extracting nucleic acids from the cell,
a DNase treatment step of degrading DNA of the extracted nucleic acids with DNase,
a step of hybridizing mRNA of the total RNA contained in the single-cell with oligo (dT) fixed onto a carrier,
a step of performing reverse transcription of the mRNA hybridized with the oligo (dT) to fix cDNA derived from the single-cell onto the carrier, thereby preparing a single-cell derived cDNA library fixed onto a carrier, and
a step of amplifying cDNA fixed onto the carrier and simultaneously detecting an amplification amount of the cDNA. |
