发明名称 |
Production of weakly active or inactive mutants of alkaline phosphatase and their expression in yeast |
摘要 |
The invention concerns a method for the recombinant production or expression of eukaryotic alkaline phosphatase mutants in yeast cells wherein the specifically introduced mutations result in a reduction of the specific AP activity by at least a factor 1:100. The invention also concerns a method for inserting corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it concerns corresponding vectors and host strains.
|
申请公布号 |
US7303903(B2) |
申请公布日期 |
2007.12.04 |
申请号 |
US20070712877 |
申请日期 |
2007.03.01 |
申请人 |
ROCHE DIAGNOSTICS OPERATIONS, INC. |
发明人 |
MUELLER RAINER;THALHOFER JOHANN-PETER;GEIPEL FRANK;HOELKE WERNER;KIRSCHBAUM THOMAS |
分类号 |
C12N15/09;C12P21/06;C07H21/04;C07K14/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/16;C12N15/00;C12N15/55;C12R1/84 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|