| 摘要 |
The present invention is drawn to methods and compositions for the detection and/or quantification of nucleic acid encoding alpha-amylase in a sample. Provided herein are novel primer and probe compositions for use in detecting the presence of alpha-amylase in an organism, particularly using quantitative PCR methods. Provided herein are novel oligonucleotide primers and probes, including the primers set forth in SEQ ID NO:3 and 4 and the novel oligonucleotide probe sequence set forth in SEQ ID NO:5, including variants, fragments and complements thereof, kits comprising these primer and probe sequences, and methods for using these primers and probes for the detection and/or quantification of alpha-amylase in an organism, particularly a genetically-modified organism.
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