发明名称 |
METHOD FOR THE SIMULTANEOUS DETECTION OF MUTATIONS OCCURRING IN RELATED GENOMES, EXPLOITING MOBILITY VARIATIONS IN 2-D ELECTROPHORESIS OF DNA DUPLEXES UNDERGOING DIFFERENTIAL HELIX-COIL TRANSITION IN A DENATURING GRADIENT |
摘要 |
A method for the simultaneous mutation detection exploiting the variable electrophoretic mobility of related DNA samples, caused by the differential onset of helix-coil transition in denaturing gradients, comprises the following subsequent steps: a) preparation of DNA samples; b) fragmentation of the samples through a first restriction; C) terminal labelling of the fragments of each genome with a different fluorochrome; d) mixing the samples; e) carrying out a simultaneous second restriction, converting each long duplex into a set of shorter fragments with two of them labelled at only one of their ends e) carrying out a 2D separation of the mixed digestion products in order to obtain: a first dimension consisting of a standard 20 polyacrylamide gel; and a second dimension against a denaturing gradient; f) spotting, picking of singly labelled spots and sequencing of their DNA. |
申请公布号 |
WO2007085882(A3) |
申请公布日期 |
2007.11.22 |
申请号 |
WO2006IB00266 |
申请日期 |
2006.01.27 |
申请人 |
PARCO TECNOLOGICO PADANO SRL;SGARAMELLA, VITTORIO;PANELLI, SIMONA;DAMIANI, GIUSEPPE |
发明人 |
SGARAMELLA, VITTORIO;PANELLI, SIMONA;DAMIANI, GIUSEPPE |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
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