AUTOMATABLE ARTIFICIAL IMMUNE SYSTEM (AIS)

摘要

The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. In particular the claimed artificial immune system comprises a vaccination site, comprising a first matrix and a plurality of cells attached to said matrix, wherein a plurality of said vaccination sites are organized in a multi-well format; and a three-dimensional artificial lymphoid tissue, comprising a second matrix and a plurality of lymphocytes and leukocytes, wherein a plurality of said three-dimensional artificial lymphoid tissues are organized in a second multi-well format. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system.