| 摘要 |
<p>The invention is directed to a method for producing precursor cells expressing a target gene, for example VEGF, at desired levels comprising stably transducing precursor cells with the target gene linked to a cell-surface marker gene, for example truncated CD8/CD8a, via an IRES sequence accommodating translation of the target and the marker genes from the same mRNA, then correlating an expression level of the target gene with that of the cell-surface marker gene, selecting a reference clone of cultured precursor cells expressing the target gene at the desired threshold, and isolating precursor cells having the same or similar level of expression of the target gene than the reference clone.</p> |
