发明名称 |
Methods of identifying synthetic transcriptional and translational regulatory elements, and compositions relating to same |
摘要 |
Provided are methods of identifying oligonucleotides having transcriptional or translational activity by integrating ilie oligonucleotide into a eukaryotic cell genome such that the oligonucleotide is operatively linked to an expressible polynucleotide, and detecting a change in expression of the expressible polynucleotide due to the operatively linked oligonucleotide. Also provided are vectors useful for identifying an oligonucleotide having transcriptional or translational regulatory activity according to a method of the invention. In addition, isolated synthetic transcriptional or translational regulatory elements identified according to a method of the invention are provided, as are kits, which contain a vector useful for identifying a transcriptional or translational regulatory element, or an isolated synthetic transcriptional or translational regulatory element or plurality of such elements. Also provided are isolated transcriptional regulatory elements.
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申请公布号 |
US2007270580(A1) |
申请公布日期 |
2007.11.22 |
申请号 |
US20060607131 |
申请日期 |
2006.11.29 |
申请人 |
THE SCRIPPS RESEARCH INSTITUTE |
发明人 |
MAURO VINCENT P.;EDELMAN GERALD M.;CHAPPELL STEPHEN A.;JONES FREDERICK S.;OWENS GEOFFREY;MEECH ROBYN |
分类号 |
C07H21/04;C12N15/00;C12N15/10;C12N15/79;C12N15/867;C12N15/90;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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