| 摘要 |
A novel affinity capture surface, as well as methods of using and making the affinity capture surface and sample presentation devices or biochips comprising the affinity capture surface, are disclosed. The affinity capture surface comprising a substrate surface having adjacent first and second affinity capture zones, wherein the first affinity capture zone comprises a first binary self-assembled monolayer comprising a plurality of affinity capture monomers and hydrophilic-terminated monomers associated with the substrate surface and the second affinity capture zone comprises a second binary self-assembled monolayer comprising a plurality of affinity capture monomers and hydrophobic-terminated monomers associated with the substrate surface, and wherein the affinity capture monomers are capable of selectively retaining an analyte and are cleavable to release terminal portions of the affinity capture monomers and the analyte, thereby generating a hydrophilic surface in the first affinity capture zone and a hydrophobic surface in the second affinity capture zone. |
