发明名称 |
NATURAL TISSUE-DERIVED DECELLULARIZED MATRIX AND METHODS OF GENERATING AND USING SAME |
摘要 |
<p>Decellularized tissue-derived extracellular matrices (ECM) and methods of generating and using same are provided. The method of generating a decellularized matrix includes the steps of: (a) subjecting the tissue to washes and a hypertonic buffer; (b) subjecting the tissue to an enzymatic proteolytic digestion with an enzyme such as trypsin; and (c) removing all cellular components from the tissue using a detergent solution which includes Triton-X-100 and ammonium hydroxide. Specifically, there is provided a decellularized myocardium-derived matrix which is completely devoid of all cellular components and hence non-immunogenic in a subject, exhibits suitable structural and mechanical properties for cardiac tissue engineering or replacement therapy of damaged cardiac tissue, and is capable of remodeling upon seeding of cells.</p> |
申请公布号 |
EP1856246(A2) |
申请公布日期 |
2007.11.21 |
申请号 |
EP20060711286 |
申请日期 |
2006.03.07 |
申请人 |
TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED |
发明人 |
MACHLUF, MARCELLE;EITAN, YUVAL |
分类号 |
A61L27/36 |
主分类号 |
A61L27/36 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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