METHOD FOR REGENERATION OR TRANSPLANTATION OF CARTILAGE

摘要

The presence of a cartilage progenitor cell in the auricular cartilaginous membrane in an adult rabbit is shown by the labeling with 5-bromo-2'-deoxyuridine, and the clone forming ability and the differentiation of the cell are analyzed. The presence of a long-term label-retaining cell has been demonstrated in the cartilaginous membrane. A cell derived from a cartilaginous membrane (i.e., a cartilaginous membrane cell) is separated physically using a scalprum and maintained in D-MEM/F-12 culture medium supplemented with 10% FCS. The resulting cartilaginous membrane cell shows a more excellent growth capability than a cartilage cell. The cartilaginous membrane cell can be differentiated into a fat cell and a bone forming cell in an induction medium.