BUFFERS FOR DETECTION OF MRNA SEPARATED IN A MICROFLUIDIC DEVICE

摘要

The present invention provides methods and kits using low conductivity buffers for the detection of mRNA from a cell lysate separated in a microfluidic device. The buffers do not affect the integrity of the cell nucleus, thereby allowing for generation of a cell lysate that can be directly used as a template in reverse transcriptase (RT)-polymerase chain reaction (PCR) amplification reactions without contaminating genomic DNA.