| 摘要 |
A procedure for regenerating cartilage in a patient's joint damaged by Osteoarthritis by transplanting autologous hematopoetic stem cells extracted from a human, preferably the same human. The procedure begins by screening to ensure that the patient is a suitable candidate, then preparation of the patient for the stem cell transplantation by a combined regimen of intra-articular injection to diminish inflammation and facilitate apoptotic cell clearance, plus subcutaneous injection of hematopoetic growth factor. The autologous hematopoetic stem cells are then purified by incubating a blood sample with magnetic polystyrene beads (Dynabeads(TM)) coated with a monoclonal antibody specific for CD34 cell membrane antigen. The stem cells are separated from the blood sample using a magnetic particle separator, and washed through a plurality of washing substeps. The purified and separated stem cells are suspended in a particular transplant solution, and the transplant solution is injected into the patient's joint such that the purified and separated autologous hemotopoetic stem cells enter the patient's damaged joint, and regenerates. The transplant solution contains specific growth factors that direct the stem cells into the areas of damaged or absent cartilage, allowing the stem cells to differentiate into young chondrocytes. This produces new healthy cartilage with a high potential in restoring the integrity and functionality of the joint. |
