Method for Detecting Dna Point Mutations (Single Nucleotide Polymorphism (Snp) Analysis) and Associated Arrangement

摘要

The single nucleotide polymorphism analysis involves the utilization of a DNA hybridization as well as the use of a DNA chip. A liquid DNA sample to be analyzed is guided over a DNA chip in a defined time course. After successful hybridization, the temperature is modified in a defined manner under low stringency conditions do that scavenger/target DNA hybrids are melted, whereby the melting of the scavenger/target DNA hybrids is detected and evaluated according to the temperature. In addition to the DNA chip, at least one device is provided for controlling or regulating temperature, and a device is provided for the lateral flowing against of the surface of the DNA chip. Factors for matching hybrids and mismatching/single point mismatching hybrids can be detected and evaluated using appropriate measuring device(s).