METHOD FOR QUANTITATIVE PCR DATA ANALYSIS SYSTEM (QDAS)

摘要

This invention provides a method for the quantitation and quality assurance of QPCR data. In particular, the invention provides a method for extracting data curves from a QPCR assay, estimating the shape and characteristics of an amplification curve and producing a quality score metric. The invention also provides a robust method for calculating a threshold cycle (CT) value and classifying the status of the QPCR results.