| 摘要 |
PROBLEM TO BE SOLVED: To provide a cell fusion device for efficiently carrying out a cell fusion with a pair of two types of cells, and to provide a method for the cell fusion using the device. SOLUTION: The cell fusion device includes a pair of electrodes formed by an electrically conducting member and disposed opposite to each other in the cell fusion region in which the cell fusion is carried out, a cell fusion container formed by flat plate-like electrical insulators disposed via a flat plate-like spacer in between the pair of electrodes and provided with one or more fine pores penetrated in the direction of the electrodes disposed opposite to each other, and an electric source for applying an alternating voltage to the pair of electrodes. The method for the cell fusion using the device comprises the following practice: A first cell is introduced into the cell fusion region followed by application of an alternating voltage with the above-mentioned waveform to immobilize the first cell in the fine pore(s). Thereafter, a second cell is introduced into the cell fusion region followed by application of an alternating voltage with the above-mentioned waveform to contact the second cell with the first cell at the position of the fine pore(s) to accomplish the objective cell fusion chemically. COPYRIGHT: (C)2008,JPO&INPIT |
