| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a rapid and effective method of producing a reovirus in cultured cells for production of reovirus suitable for clinical administration. <P>SOLUTION: The present invention is directed to a method of extracting virus, particularly retrovirus, from a culture of cells. Infectious virus can be extracted from the culture with a detergent at a convenient temperature such as 25°C or 37°C to produce high virus titers. Both ionic and non-ionic detergents can be used in the present invention. In a preferable embodiment, the virus is a non-enveloped virus. The non-enveloped virus is preferably a reovirus. The present invention is applicable to any reoviruses, preferably a mammalian reovirus. <P>COPYRIGHT: (C)2008,JPO&INPIT</p> |
