| 摘要 |
A composition comprising dual marker-modified siRNA(small interfering RNA) is provided to determine an amount of siRNA accurately and effectively through immunoassay method, thereby safely and accurately measuring in vivo distribution and blood half-life of siRNA in pharmacokinetic studies. A quantitative composition comprises dual marker-modified siRNA, wherein one marker is useful for plate binding for immunoassay and another marker is for recognition of a first antibody for immunoassay. The marker for plate binding is selected from glutathione-s-transferase, beta-galactosidase, spermidine, dopamine, prostaglandin, dinitrophenol, sugar and biotin and the marker for recognition of a first antibody is histidine, c-myc, flag, haemagglutinin(HA), glutathione-s-transferase, beta-galactosidase, spermidine, dopamine, prostaglandin and dinitrophenol. A quantification method of siRNA comprises the steps of: reacting the quantitative composition in a plate coated with a protein capable of binding the plate-binding marker for a certain time; and treating the plate with a first antibody specifically recognizing the first antibody-recognizing marker.
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