METHOD OF REAL TIME DETECTION OF PATHOGENIC MICROORGANISM USING THE COMPETITIVE IMMUNOREACTION-MEDIATED FLOW TYPE SURFACE PLASMON RESONANCE BIOSENSOR

摘要

A method for real time detection of pathogenic bacteria by using a competitive immunoreaction-mediated flow type surface plasmon resonance(SPR) biosensor is provided to improve ability for detecting low concentration of waterborne pathogenic bacteria including Cryptosporidium parvum without labeling. A method for real time detection of pathogenic bacteria comprises the steps of: (i) immunoreacting a pathogenic bacterium with antibodies thereof(first antibody) in a batch type; (ii) selectively separating unreacted antibodies by using cut-off membrane filter; (iii) binding the separated unreacted antibodies to a second antibody immobilized to the surface of a flow type surface plasmon resonance(SPR) system chip in real time; and (iv) continuously collecting the real time SPR signal generated from the surface of flow type SPR system chip of unreacted antibody.