MODIFIED CREATININE AMIDE HYDROLASE HAVING IMPROVED AFFINITY FOR SUBSTRATE, AND REAGENT COMPOSITION FOR DETERMINATION OF CREATININE

摘要

[PROBLEMS] To overcome disadvantages of a known creatinine amide hydrolase, and provide a creatinine amide hydrolase having improved affinity for creatinine or having a decreased Km value for creatinine, and also provide a reagent composition for use in the determination of creatinine, which is adapted to an automated analysis apparatus and is excellent in accuracy, preciseness and economic efficiency. [MEANS FOR SOLVING PROBLEMS] Disclosed is a modified creatinine amide hydrolase having improved affinity for a substrate compared to an unmodified one. Also disclosed is a creatinine determination reagent comprising the modified creatinine amide hydrolase, a creatinine amidino hydrolase, sarcosine oxidase and a reagent for detection of hydrogen peroxide.