METHOD FOR PRODUCING 5-NORBORNEN-2-CARBOXYLIC ACID FROM 5-NORBORNEN-2-CARBONITRILE USING AN ARYLACETONITRILASE

摘要

The present invention relates to a method for producing 5-norbornen-2-carboxylic acid from 5-norbornen-2-endo-carbonitrile and/or 5-norbornen-2-exo-carbonitrile. The invention relates more specifically to a method for producing 5-norbornen-2-carboxylic acid at a high substrate concentration. Furthermore, the invention relates to a polypeptide suitable for the enzymatic reaction of 5-norbornen-2-carbonitrile to 5-norbornen-2-carboxylic acid, particularly also at a high substrate concentration, as well as to a nucleic acid coding for the polypeptide, a composition containing 5-norbornen-2-carbonitrile to 5-norbornen-2-endo-carboxylic acid and 5-norbornen-2-exo-carboxylic acid, as well as the use of the polypeptide.