| 摘要 |
A stabilising diluent for a protein-horseradish peroxidase conjugate for use in an enzyme immunoassay for the detection of a human Parvovirus B19 antibody, comprises stabilising amounts of bovine serum albumin (BSA), cytochrome c and gelatin hydrolysate, an antimicrobial agent consisting of 2-methyl-4-isothiazolin-3-one and 5-bromo-5-nitro-1, 3-dioxane, a non-ionic surfactant and a biological buffer, such that the diluent has a pH in the range 7.0 to 7.5. Use of the stabilising diluent results in the protein-horseradish peroxidase conjugate retaining at least 70% reactivity following accelerated stress testing at a temperature in the range 35-39{C for a period of at least 4 days relative to a positive control at a temperature in the range 2-8{C. |
