| 摘要 |
<p>A mutant Pichia pastoris alcohol oxidase 1 (AOXl) promoter of the wild type Pichia pastoris AOXl promoter (SEQ ID No. 1) comprising at least one mutation selected from the group consisting of : a) a transcription factor binding site (TFBS), b) nucleotides 170 to 235 (-784 to-719), nucleotides 170 to 191 (-784 to-763), nucleotides 192 to 213 (-762 to-741), nucleotides 192 to 210 (-762 to-744), nucleotides 207 to 209 (-747 to-745), nucleotides 214 to 235 (-740 to-719), nucleotides 304 to 350 (-650 to-604), nucleotides 364 to 393 (-590 to-561), nucleotides 434 to 508 (-520 to-446), nucleotides 509 to 551 (-445 to-403), nucleotides 552 to 560 (-402 to-394), nucleotides 585 to 617 (-369 to-337), nucleotides 621 to 660 (-333 to-294), nucleotides 625 to 683 (-329 to-271), nucleotides 736 to 741 (-218 to-213), nucleotides 737 to 738 (-217 to-216), nucleotides 726 to 755 (-228 to-199), nucleotides 784 to 800 (-170 to-154) or nucleotides 823 to 861 (-131 to-93) of Seq ID No. 1, and combinations thereof.</p> |
