| 摘要 |
There is provided a method for detecting a reaction of a fluorescently labeled protein and a sample simply, in a short time and at a higher precision. By using an expression system such as an extracellular gene expression system and an extracellular transcription or translation system, a reaction of expressing a protein from a constructed vector is performed. At this time, a fluorescently labeled amino acid is introduced into the expression system in the form of a tRNA having the fluorescently labeled amino acid. With expression of a protein, the fluorescently labeled amino acid is incorporated into the protein, and a fluorescently labeled protein (hereafter, referred to as fluorescently labeled protein) is produced. The fluorescently labeled protein and a sample S are mixed to prepare a mixed solution, and FCS measurement is performed. Based on a measured value of the FCS measurement, the presence or absence of a reaction is detected.
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