AN ANTIOXIDANT COMPOSITION COMPRISING 4-HYDROXYPINORESINOL, WHICH HAS PROTECTIVE ACTIVITY AGAINST OXIDATIVE STRESS IN BRAIN TISSUE AND LIVER TISSUE, AS AN EFFECTIVE INGREDIENT

摘要

A composition comprising 4-hydroxypinoresinol as an effective ingredient is provided to inhibit neurotoxicity and oxidative stresses in brain tissue and suppress oxidative stresses in liver tissue, thereby protecting brain and liver tissues. A composition for protecting brain and liver tissues comprises 4-hydroxypinoresinol represented by the formula(1) derived from petaslignolide A contained in bog rhubarb as an effective ingredient, wherein the 4-hydroxypinoresinol is isolated from petaslignolide A by treating petaslignolide A with naringinase or glycosidase to obtain 4-hydroxypinoreinol, solubilizing the 4-hydroxypinoreinol in DMSO(dimethyl sulfoxide) and acidic buffer sequentially, adding excess naringinase into the solution and fractionating the solution with ethyl acetate to obtain an ethyl acetate layer, concentrating the ethyl acetate layer and subjecting it to TLC(thin layer chromatography).