BIOPOLYMER SYNTHESIS APPARATUS, METHOD FOR SYNTHESIZING BIOPOLYMER AND METHOD FOR FABRICATING MICROARRAY

摘要

A biopolymer synthesizing device is provided to allow all steps of the polymer synthesis to be continuously done in a closely sealed chamber, thereby increasing the process efficiency and preventing the contamination by the foreign materials flowing in. A biopolymer synthesizing device(600) comprises at least one chamber(200) including a chamber body and a chamber cover covering the chamber body and having at least one through hole on the upper side; a fluid supplying pipe(320) connected to the chamber through the through hole; and a drain part(400) connected to a drain hole formed on the lower side of the chamber to which a nucleotide phosphoramidite monomer of which a photodegradable protecting group is coupled is supplied through the fluid supplying pipe, wherein the nucleotide is a nucleotide having one base selected from the group consisting of adenine, guanine, thymine and cytosine. A method for preparing a microarray comprises the steps of: (a) providing a substrate having a functional group able to react with a nucleotide phosphoramidite monomer; (b) providing a nucleotide phosphoramidite monomer of which a photodegradable monomer is coupled to the substrate to be coupled to the functional group; (c) capping the functional group not reacting with the monomer; and (d) oxidizing the monomer coupled to the functional group, wherein the coupling, capping and oxidizing are performed in a closely sealed single chamber.