| 摘要 |
<p>A method for genetically discriminating Porphyra yezoensis Ueda is provided to discriminate the Porphyra yezoensis species more accurately and conveniently through the sequence analysis. A method for genetically discriminating Porphyra yezoensis Ueda comprises the steps of (a) determining a sequence of an upstream intron of SSU rDNA of the Porphyra yezoensis Ueda and standing it in line with a sequence of an upstream intron of SSU rDNA of a Japanese Porphyra yezoensis and (b) determining it to be a native species if at least one base at position 12, 71, 75, 82, 251, 331, and 348 is A or at least one base at position 343 and 495 is A or at least one base at position 353, 356, and 496 is G, or at least one base at position 485 and 486 is G. Before the step(a), the upstream intron of the SSU rDNA of Porphyra yezoensis is amplified through PCR using a primer set of InF1(sequence 2) and InR1(sequence 3).</p> |
