| 摘要 |
The invention relates to methods for generating insulin secreting cells from precursor stem cells or from adult pancreatic exocrine cells by exposing them to a nucleic acid molecule encoding neurogenin3 (ngn3) or NeuroD/ ß2. The methods of the invention are useful, for example, for generation of glucose sensitive insulin-secreting ß-cells suitable for transplantation, as well as for in situ development of insulin-secreting cells in a patient in need thereof. Further, the method of the invention relates to methods for preventing premature differentiation of precursor stem cells into insulin-secreting ß-cells. Still further, the invention relates to assay methods for identification of compounds that prevent or activate ß-cell differentiation.
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