| 摘要 |
<p>The present invention relates to a multiparallell method for construction of host /vector- systems, comprising transforming a plurality of host strains, wherein substantially all host strains have deleterious mutations in at least one uptake system for a critical substrate needed for the growth of said host strain, with a plurality of vectors encoding at least one protein, and expressing said recombinant protein in said host strains. It further relates to host/vector systems obtainable by the method, to kits comprising the host strains and optionally the vectors, and to use of produced host/vector systems for protein expression. The method is exemplified using an E. coli strain with deleterious mutation in both ptsG and ptsM.</p> |
