| 摘要 |
A reagent for testing the coagulability of blood during treatment with anticoagulent agents comprises thromboplastin in optimal concentration having a Quick's thromboplastin time of at least 35 seconds with normal human plasma in admixture with cephalin or a cephalin substitute, e.g. lecithin. Bovine plasma from which prothrombin, Stuart-factor, proconvertin and antihemophilia-B-factor have been removed and which contains high concentrations of antihemophilia-A and -C-factors, Hagemanfactor, proaccelarin and proconvertin, and calcium chloride may also be present, the latter in an amount sufficient to give the optimal concentration of Ca/sR/sR ions for coagulation after the addition of test plasmas. The reagent may be freeze-dried to give a stable, solid preparation which may be reconstituted before use. The prothrombin may be prepared by known procedures from animal (e.g. bovine, horse, swine) brain and the cephalin from human brain. The bovine plasma may be obtained by the treatment thereof with barium sulphate followed by dialysis to remove oxalate and the carbonic acid/bicarbonate buffer system and adjustment of the pH to from 6-8.ALSO:A reagent for testing the coagulability of blood during treatment with anticoagulent agents comprises thromboplastin in optional concentration having a Quick's thromboplastin time of at least 35 seconds with normal human plasma in admixture with cephalin or a cephalin substitute e.g. lecithin. Bovine plasma from which prothrombin, Stuart-factor, proconvertin and antihaemophilia-b -factor have been removed and which contains high concentrations of antihaemophilia-A and -C-factors, Hageman-factor, proaccelarin and proconvertin, and calcium chloride may also be present, the latter in amounts sufficient to give the optimal concentration of CA++ ions for coagulation after the addition of test plasma. The reagent may be freeze-dried to give a stable, solid preparation which may be reconstituted before use. The prothrombin may be prepared by known methods from animal (e.g. bovine, horse, swine) brain and the cephalin from human brain. The bovine plasma may be obtained by the treatment thereof with barium sulphate followed by dialysis to remove oxalate and the carbonic acid/bicarbonate buffer system and adjustment of the pH to from 6 to 8. |
