发明名称 |
NON-HUMAN CHIMERIC ANIMAL AND USE THEREOF |
摘要 |
This invention provides: a pluripotent cell derived from a non-human animal comprising foreign DNA that encodes a desired protein in such a manner that the expression of the desired protein is regulated by the control region of a gene expressed in certain cells and/or tissue, wherein the foreign DNA is bound to a nucleic acid fragment comprising a promoter/the whole or part of 5' non-translational region/a leader sequence coding region derived from a gene expressed in certain cells and/or tissue, and wherein in said cell one or more drug resistant marker genes used for introducing the foreign DNA into the genome have been removed; a chimeric non-human animal that is prepared from the pluripotent cell and highly expresses the desired protein, or a progeny thereof; a method for producing a desired protein using the chimeric animal; and a method for analyzing in vivo function of a desired gene using the chimeric animal.
|
申请公布号 |
EP1849863(A1) |
申请公布日期 |
2007.10.31 |
申请号 |
EP20060701439 |
申请日期 |
2006.01.23 |
申请人 |
KIRIN PHARMA KABUSHIKI KAISHA |
发明人 |
KAKITANI, MAKOTO;TOMIZUKA, KAZUMA |
分类号 |
C12N15/13;C12N15/16;C12N15/62;C12N15/67;C12P21/02;C12N5/10;A01K67/027 |
主分类号 |
C12N15/13 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|