| 摘要 |
A therapeutic preparation effective against tumours and microbial infections consists of a lysate or lysate fraction of a killed Bacillus subtilis type mesentericus QM-B1585. The microorganism may be cultured in aerobic submerged culture at 37 DEG C. in an aqueous medium containing peptone and saccharose. The bacteria are separated from the medium by (a) centrifugation, (b) filtration or (c) addition of acetone. The separated bacteria are killed and dehydrated by means of 20 volumes of acetone at -20 DEG C. followed by ether at -20 DEG C. They are then dried. The dried bacteria are subjected to lysis by treatment with (a) lysozyme in physiological saline at 37 DEG C. for 35 minutes and then (b) a protease such as trypsin for 1 hour at 37 DEG C. An active fraction may be precipitated from the aqueous lysate by addition of (a) a copper salt such as CuSo4, (b) acetone or (c) cetyl trimethyl ammonium bromide. The copper ions are removed from the copper complex by adsorption on a strong cation-exchange resin. The preparation is administered as the active fraction of the lysate, the aqueous lysate or the dried lysate.
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