Method for the determination of the composition of textile fibers

摘要

Method for the recognition and the quantitative evaluation of mixed textile fibers of animal source, comprising the steps of: preparing and conditioning of the fibers to be analysed; extracting of the proteins by means of demolition of the fibers, and subsequent centrifugation; dosing the mixture of proteins contained in the fibers; separing by electrophoresis the mixture of proteins; transferring the proteins on a membrane and reacting with at least a specific monoclonal antibody; marking with an enzyme conjugated secondary antibody; the said enzyme reacting with a substrate so as to provide a chemiluminescent product; detecting the chemiluminescence by means of a photoplate and; scanning and analysing of the obtained images by means of a processor provided with a suitable program.