The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.
申请公布号
WO2007120208(A2)
申请公布日期
2007.10.25
申请号
WO2006US44209
申请日期
2006.11.14
申请人
PRESIDENT AND FELLOWS OF HARVARD COLLEGE;CHURCH, GEORGE, M.;PORRECA, GREGORY, J.;SHENDURE, JAY;ROSENBAUM, ABRAHAM
发明人
CHURCH, GEORGE, M.;PORRECA, GREGORY, J.;SHENDURE, JAY;ROSENBAUM, ABRAHAM