发明名称 |
BI-DIRECTIONAL SELECTION MARKERS WITH IMPROVED ACTIVITY |
摘要 |
<p>The present invention discloses a polypeptide selected from the group consisting of: a polypeptide having an amino acid sequence according to SEQ ID NO 3, a polypeptide having an amino acid sequence according to SEQ ID NO 6, a polypeptide having an amino acid that is substantially homologous to the sequence of SEQ ID NO 3 and a polypeptide having an amino acid that is substantially homologous to the sequence of SEQ ID NO 6, the polypeptide displaying acetamidase activity and providing a reverse selection on fluoroacetamide with an efficiency of at least 95%, preferably at least 96%, more preferably at least 97%, more preferably at least 98%, more preferably at least 99%, most preferably 100%. The gene encoding the polypeptide of the invention is used as an efficient bi-directional selection marker in the construction of selection marker free strains, in particular for processes for the production of a compound of interest.</p> |
申请公布号 |
WO2007118836(A1) |
申请公布日期 |
2007.10.25 |
申请号 |
WO2007EP53549 |
申请日期 |
2007.04.12 |
申请人 |
DSM IP ASSETS B.V.;BERG, VAN DEN, MARCO ALEXANDER;BOVENBERG, ROELOF, ARY, LANS |
发明人 |
BERG, VAN DEN, MARCO ALEXANDER;BOVENBERG, ROELOF, ARY, LANS |
分类号 |
C12N15/55;C12N9/80 |
主分类号 |
C12N15/55 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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