Compositions and methods for the detection of a nucleic acid using circular probes in a cleavage reaction

摘要

The invention provides a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample by forming a cleavage structure by incubating a sample comprising a target nucleic acid sequence with a probe, nucleic acid polymerase, nuclease and optionally a primer. The target nucleic acid is extended and circularized and a cleavage structure is formed. The cleavage structure is cleaved with a nuclease to generate a nucleic acid fragment which is indicative of the presence of a target nucleic acid sequence in the sample.