| 摘要 |
The present invention uses the principle that phosphomolybdate binds to hydrophobic surfaces to isolate the phos-phomolybdate complex from other phosphate-containing molecules and further uses the SPA concept to bring a radiolabeled phos-phomolybdate complex in close contact with a scintillant for measuremen t by scintillation counting. Generally, the present invention provides an assay for detecting and measuring the amount of orthophosphate (Pi) in an aqueous reaction mixture, wherein the amount of Pi released is separated from the reaction mixture by: adding a solution of molybdate to the reaction mixture to form a phosphomolybdate complex; and contacting the phosphomolybdate complex with a hydrophobic surface, wherein the surface is capable of being separated from the aqueous reaction mixture to allow measurement of th e Pi. Particularly, this invention provides an assay for measuring the ATPase activity of enzymes, more particularly, the HPV El helicase. |
