| 摘要 |
Methods for obtaining nucleic acid from nucleated cells are disclosed, wherein RNA and fragmented genomic DNA can be sequentially obtained from the same starting material are disclosed. In some embodiments, protein can also be obtained from the same starting material as the RNA and DNA are obtained. According to certain methods, whole blood or a blood fraction comprising nucleated cells is combined with a capture surface and at least some of the leukocytes are retained on the surface. The RNA is released from the retained leukocytes, then the capture surface is treated with a suitable nuclease or other DNA fragmenting agent to release DNA fragments. In certain embodiments, either the released RNA, the released DNA, or both the released RNA and the released DNA are employed in one or more molecular biology application.
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