RING DNA MOLECULE WITH CONDITION-DEPENDENT REPLICON ORIGIN, METHOD FOR PRODUCTION THEREOF AND USES IN GENE THERAPY

摘要

FIELD: biotechnology. ^ SUBSTANCE: disclosed is procariotic recombinant host cell containing heterologous protein of replication activation, which activated condition-dependent replicon origin, and extrachromosomal DNA molecule, containing heterologous therapeutic gene and condition-dependent replicon origin. Also disclosed are method for producing of plasmid containing heterologous therapeutic gene and condition-dependent replicon origin, plasmid representing suicide vector for gene transferring, containing heterologous therapeutic gene and condition-dependent replicon origin. ^ EFFECT: method for avoiding of non-controlled overexpression of therapeutic gene and resistance genes. ^ 44 cl, 41 dwg, 10 tbl, 14 ex