多点置换的蛋白酶变体

摘要

公开了由天然存在的或重组的非人类蛋白酶的DNA序列衍生的新型蛋白酶变体。变异蛋白酶的获得方法通常是，对编码天然存在的或重组的蛋白酶的前体DNA序列进行体外修饰，在前体蛋白酶的氨基酸序列中引起多处氨基酸残基的置换。这种变体蛋白酶具有与前体蛋白酶不同的性质，如改变的洗涤性能。置换的氨基酸残基相应于解淀粉芽孢杆菌枯草杆菌蛋白酶位置62、212、230、232、252和257。

主权项

1.一种蛋白酶变体，其为在相应于选自解淀粉芽孢杆菌枯草杆菌蛋白酶232残基位置的氨基酸置换的如下蛋白酶变体：V68AN76DS103AV104IG159DA232VQ236HQ245R，N76DS103AV104IA232VQ245R，S24TV68AN76DS103AV104IG159DA232VQ236HQ245R，V68AS103AV104IG159DA232VQ236HQ245RN252K，V68AN76DS103AV104IG159DT213RA232VQ236HQ245RT260A，V68AS103AV104IG159DA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DA232VQ236HQ245R，V68AS103AV104IN140DG159DA232VQ236HQ245RN252K，N43SV68AS103AV104IG159DA232VQ236HQ245RN252K，N43KV68AS103AV104IG159DA232VQ236HQ245R，N43DV68AS103AV104IG159DA232VQ236HQ245RN252K，V68AS87GS103AV104IG159DA232VQ236HQ245RN252KR275S，V68AS103AV104IG159DA232VQ236HQ245RL257V，V68AS103AV104IN116DG159DA232VQ236HQ245R，V68AS103AV104IG159DA232VQ236HQ245RN248D，R10CV68AS103AV104IG159DA232VQ236HQ245R，V68AS103AV104IG159DV203EA232VQ236HQ245R，V68AS103AV104IG159DA232VQ236HK237EQ245R，V68AN76DI79NS103AV104IG159DA232VQ236HQ245R，V68AS103AV104IG159DN183DA232VQ236HQ245R，V68AS103AV104IG159DA174VQ206LA232VQ236HQ245R，V68AS103AV104IG159DS188CA232VQ236HQ245R，V68AS103AV104IG159DA230TA232VQ236HQ245R，V68AA98TS103AV104IG159DA232VQ236HQ245R，V68AS103AV104IG159DA215TA232VQ236HQ245R，V88AS103AV104IG159DA232VQ236HQ245RN248S，V68AN76DS103AV104IG159DA232VQ236HQ245R，V68AN76DS103AV104IG159DP210RA232VQ236HQ245R，V68AN76DS103AV104IG159DA232VQ236HQ245RL257V，N76DS103AV104IA232VQ236HQ245RL257V，V68AS103AV104IG159DA232VQ236HQ245RL257VR275H，V68AS103AV104IG159DT224AA232VQ236HQ245RL257V，N76DS103AV104IG159DA232VQ236HQ245RL257V，V68AN76DS103AV104IG159DY209WA232VQ236HQ245R，V68AN76DS103AV104IG159DG211RA232VQ236HQ245R，V68AN76DS103AV104IG159DG211VA232VQ236HQ245R，Q12RV68AN76DS103AV104IG159DY214LA232VQ236HQ245R，V68AN76DS103AV104IG159DA215RA232VQ236HQ245R，Q12RV68AN76DS103AV104IG159DA232VQ236HQ245R，G20RV68AN76DS103AV104IG159DA232VQ236HQ245RS259G，V68AS87RN76DS103AV104IG159DA232VQ236HQ245RT260V，V68AN76DS103AV104IG159DA232VQ236HQ245RN261G，V68AN76DS103AV104IG159DA232VQ236HQ245RN261W，N76DS103AV104IA232VQ236HS242PQ245R，V68AN76DS103AV104IG159DP210LA232VQ236HQ245R，Q12RA48VV68AN76DS103AV104IG159DA232VQ236HQ245R，N76DS103AV104IA232VQ236HQ245R，N76DS103AV104IG159DY192FA232VQ236HQ245R，N76DS103AV104IV147IG159DA232VQ236HQ245RN248SK251R，Q12RV68AN76DS103AV104IG159DA232VQ236HQ245RA272S，V68AN76DS103AV104IG159DN183KQ206LA232VQ236HQ245R，V68AN76DS103AV104IG159DA232VQ236HQ245RS256R，V68AN76DS103AV104IG159DQ206RA232VQ236HQ245R，K27RV68AN76DS103AV104IG159DA232VQ236HQ245R，V68AN76DS103AV104IN116TG159DR170SN185SA232VQ236HQ245R，G61EV68AS103AV104IG159DA232VQ236HQ245RN248DN252K，N43DV68AS103AV104IG159DA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DS212PA232VQ236HQ245RN248DN252K，V68AS103AV104IS99NG159DN184DA232VQ236HQ245RN248DN252K，S103AV104IG159DA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DY209WA232VQ236HQ245RN248DN252K，V68AS103AV104IQ109RG159DA232VQ236HQ245RN248DN252K，G20RV68AS103AV104IG159DA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DY209FA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DA232VQ236HQ245RN248DN252KN261D，V68AS103AV104IG159DN185DA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DP210RA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DP210TA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DP210SA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DN185DP210LA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DP210LA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DS212AA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DS212GA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DS212EA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DT213EA232VQ236HQ245RN248UN252K，V68AS103AV104IT213SA232VQ236HQ245RN248DN252K，V68AA103VV104IG159DT213EA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DT213RA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DT213GA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DA215VA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DA215RA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DS216TA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DS216VA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DS216CA232VQ236HQ245RN248DN252K，G20AV68AS103AV104IG159DA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DN173DA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DA232VQ236HQ245RN248DK251VN252K，V68AS103AV104IG159DQ206RA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DA232VQ236HQ245RN248DN252F，V68AS103AV104IG159DA232VQ236HQ245RN248DN252L，P55SV68AS103AV104IG159DA232VQ236HQ245RN248DN252F，V68AS103AV104IG159DA232VQ236HQ245RN248DN252KT255V，V68AS103AV104IG159DA232VQ236HQ245RN248DN252KS256N，V68AS103AV104IG159DA232VQ236HQ245RN248DN252KS256E，V68AS103AV104IG159DA232VQ236HQ245RN248DN252KS256R，V68AS103AV104IG159DA232VQ236HQ245RN248DN252KT260R，V68AS103AV104IG159DA232VQ236HQ245RN248DN252KL257R，V68AS103AV104IG159DA232VQ236HQ245RN248DN252KG258D，I8VV68AS103AV104IG159DA232VQ236HQ245RN248DN252KN269D，V68AS103AV104IN116SG159DA232VQ236HQ245RN248DN252KT260E，V68AS103AV104IG159DA232VQ236HQ245RN248DN252KN261R，V68AS103AV104IG159DA232VQ236HQ245RN248DN252KN261D，V68AN76DS103AV104IG159DA232VQ236HQ245RN248DN252K，V68AS103AV104IA232VQ236HQ245RN248DN252K，S103AV104IG159DA232SQ236HQ245RN248DN252K，V68AS103AV104IG159DA232VQ236RQ245RN248DN252K，N18SV68AS103AV104IG159DA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DA232VQ236HQ245VN248DN252K，V68AN76DS101TS103AV104IG159DT213RN218SA232VQ236HQ245RT260A，V68AS103AV104IG159DA228VA232VQ236HQ245RN248DN252K，T33SV68AN76DS103AV104IG159DA232VQ236HQ245RN248DN252K，V68AN76DE89DS103AV104IG159DP210LT213RA232VQ236HQ245RT260A，G61EV68AN76DS103AV104IG159DA232VQ236HQ245RN248DN252K，S103AV104IG159DV205IP210IA232VQ236HQ245R，G61EV68AS103AV104IS130AG159DA232VQ236HQ245RN248DN252K，G61EV68AS103AV104IA133SQ137RG159DA232VQ236HQ245RN248DN252K，G61ES103AV104IA133VG159DA232VQ236HQ245RN248DN252K，V68AS103AV104IG159DA232VQ236HQ245RN248GN252K，V68AS103AV104IG159DN218SA232VQ236HQ245RN248DN252K，G61EV68AS103AV104IG159DS160VA232VQ236HQ245RN248DN252K，S3LG61EV68AN76DS103AV104IA232VQ236HQ245RN248DN252K，G61EV68AS103AV104IG159DS167FA232VQ236HQ245RN248DN252K，G97ES103AV104IG159DA232VQ236HQ245RN248DN252K，A98DS103AV104IG159DA232VQ236HQ245RN248DN252K，S99ES103AV104IG159DA232VQ236HQ245RN248DN252K，S101ES103AV104IG159DA232VQ236HQ245RN248DN252K，S101GS103AV104IG159DA232VQ236HQ245RN248DN252K，G102AS103AV104IG159DA232VQ236HQ245RN248DN252K，S103AV104IS106EG159DA232VQ236HQ245RN248DN252K，S103AV104IQ109EG159DA232VQ236HQ245RN248DN252K，S103AV104IG159DA232VQ236HQ245RN248DN252KN261R，S103AV104IQ109RG159DA232VQ236HQ245RN248DN252K，N62DS103AV104IG159DA232VQ236HQ245RN248DN252K，S03AV104IG159DN184DA232VQ236HQ245RN248DN252K，S103AV104IG159DS166DA232VQ236HQ245RN248DN252K，S103AV104IG159DL217EA232VQ236HQ245RN248DN252K，G20RN62DS103AV104IG1590T213RA232VQ236HQ245RN248DN252K，N62DS103AV104IG159DT213RA232VQ236HQ245RN248DN252K，S103AV104IG159DQ206RL217EA232VQ236HQ245RN248DN252K，N62DS103AV104IG159DQ206RA232VQ236HQ245RN248DN252K，S103AV104IS130GG159DA232VQ236HQ245RN248DN252K，S103AV104IP131VG159DA232VQ236HQ245RN248DN252K，K27NS103AV104IG159DA232VQ236HQ245RN248DN252K，T38GS103AV104IG159DA232VQ236HQ245RN248DN252K，T38AN76DS103AV104IG159DT213RA232VQ236HQ245RT260A，V68AN76DS103AV104IG159DT213RA232VQ236HQ245RT260AE271G，V68AN76DS103AV104IG159DY209WT213RA232VQ236HQ245RT260A，V68AN76DS103AV104IG159DP210IT213RA232VQ236HQ245RT260A，V68AN76DS103AV104IG159DV205IT213RA232VQ236HQ245RT260A，V68AN76DS103AV104IG159DP210IA232VQ236HQ245RT260A，V68AS103AV104IG159DT213RA232VQ236HQ245RT280A，N76DS103AV104IG159DT213RA232VQ236HQ245RT260A，V68AS103AV104IG159DY209WA232VQ236HQ245R，V68AS103AV104IG159DP210IA232VQ236HQ245R，V68AS103AV104IG159DA230VA232VQ236HQ245R，V68AS103AV104IG159DL126FA232VQ236HQ245R，V68AS103AV104IG159DV205IA232VQ236HQ245R，V68AS103AV104IG159DP210LA232VQ236HQ245R，V68AS103AV104IG159DA232VQ236HQ245RT260A，S103AV104IG159DA232VQ236HQ245R，V68AS103AV104IG159DA174VA232VQ236HQ245RL257V，V68AS103AV104IG159DA194SA232VQ236HQ245RL257V，V68AS103AV104IG159DY209WA232VQ236HQ245RL257V，S103AV104IG159DA232VQ236HQ245RL257V，V68AN76DS103AV104IG159DT213RA232VQ236HQ245RT260AN261W，V68AS103AV104IG159DA232VQ236HQ245RL257VN261W，S103AV104IG159DT213RA232VQ236HQ245RT260A，S103AV104IG159DP210IA232VQ236HQ245RN248DN252K，S103AV104IG159DY209WA232VQ236HQ245RL257V，V68AN76DS103AV104IG159DP210LT213RA232VQ236HQ245RT260A，Q12RS103AV104IG159DY209WT213RA232VQ236HQ245RT260A，S103AV104IY209WA232VQ236HQ245RL257V，S103AV104IG159DV205IP210IT213RA232VQ236HQ245RT260A，S103AV104IG159DV205IY209WA232VQ236HQ245RT260A，V68AS103AV104IG159DV205IY209WP210IA232VQ236HQ245R，S103AV104IG159DV205IY209WP210IA232VQ236HQ245RL257V，S103AV104IG159DV205IY209WA232VQ236HQ245RL257V，V68AS103AV104IG159DV205IY209WP210IA232VQ236HQ245RT260A，S103AV104IG159DV205IY209WP210IA232VQ236HQ245R，S103AV104IG159DY209WP210IA232VQ236HQ245R，S103AV104IG159DV205IP210IA232VQ236HQ245R，V68AS103AV104IS128LG159DA232VQ236HQ245R，A48VV68AS103AV104IG159DY209WA232VQ236HQ245R，A48VV68AS103AV104IG159DA232VQ236HQ245RN248DN252K，A48VV68AS103AV104IG159DA232VQ236HQ245RL257VN261W，G102AS103AV104IG159DS212GA232VQ236HQ245RN248DN252K，Q12RG102AS103AV104IG159DS212GA232VQ236HQ245RN248DN252K，S101GG102AS103AV104IG159DS212GA232VQ236HQ245RN248DN252K，A98LG102AS103AV104IG159DS212GA232VQ236HQ245RN248DN252K，G102AS103AV104IG159DT213RA232VQ236HQ245RN248DN252K，S103AV104IP131VG159DA232VQ236HQ245RN248DN252K，S103AV104IG159DN184SA232VQ236HQ245RN248DN252K，S103AV104IG159DN184GA232VQ236HQ245RN248DN252K，S103AV104IG159DA232VQ236HV244TQ245RN248DN252K，S103AV104IG159DA232VQ236HV244AQ245RN248DN252K，N62DS103AV104IG159DT213RA232VQ236HQ245RN248DN252KS256R，Q12RN62DS103AV104IG159DT213RA232VQ236HQ245RN248DN252K，S101GS103AV104IG159DN185DA232VQ236HQ245RN248DN252K，S101GS103AV104IG159DQ206EA232VQ236HQ245RN248DN252K，S101GS103AV104IG159DT213QA232VQ236HQ245RN248DN252K，A98LG102AS103AV104IG159DA232VQ236HQ245RN248DN252K，S101GG102AS103AV104IG159DA232VQ236HQ245RN248DN252K，A98LG102AS103AV104IG159DS212GA232VQ236HQ245RN248DN252K，A98LG102AS103AV104IG159DS212GA232VQ236HN248DN252K，N62DS103AV104IQ109RG159DT213RA232VQ236HQ245RN248DN252K，N62DS103AV104IG159DS212GT213RA232VQ236HQ245RN248DN252K，N62DS101GS103AV104IG159DS212GT213RA232VQ236HQ245RN248DN252K，S103AV104IG159DA232VQ245RN248DN252K，N62DS103AV104IS130GG159DT213RA232VQ236HQ245RN248DN252K，S101GS103AV104IS130GG159DA232VQ236HQ245RN248DN252K，S101GS103AV104IS128GG159DA232VQ236HQ245RN248DN252K，S101GS103AV104IS128LG159DA232VQ236HQ245RN248DN252K，N62DS101GS103AV104IG159DT213RA232VQ236HQ245RN248DN252K，N62DS103AV104IS128GG159DT213RA232VQ236HQ245RN248DN252K，N62DS103AV104IS128LG159DT213RA232VQ236HQ245RN248DN252K，S101GS103AV104IG159DA232VQ236HQ245RN248DN252KT260A，S101GS103AV104IP131VG159DA232VQ236HQ245RN248DN252K，A98VS101GS103AV104IG159DA232VQ236HQ245FN248DN252K，S99GS101GS103AV104IG159DA232VQ236HQ245RN248DN252K，S101GS103AV104IG159DS212GA232VQ236HQ245RN248DN252K，S101GS103AV104IG159DY209WA232VQ236HQ245RN248DN252K，S101GS103AV104IG159DP210IA232VQ236HQ245RN248DN252K，S101GS103AV104IG159DV205IA232VQ236HQ245RN248DN252K，S101GS103AV104IG159DA194PA232VQ236HQ245RN248DN252K，N76DS101GS103AV104IG159DA194PA232VQ236HQ245RN248DN252K，S101GS103AV104IG159DA230VA232VQ236HQ245RN248DN252K，N62DS103AV104IG159DN185DQ206EA213RA232VQ245RQ245RN248DN252KE27Q。