Method of detecting bio-molecules using field effect transistor without fixing bio-molecules on the gate sensing surface

摘要

Provided is a method of detecting the presence of bio-molecules or a concentration of target bio-molecules using a field effect transistor without fixing bio-molecules on a sensing surface. The field effect transistor includes: a semiconductor substrate; a source region and a drain region which are formed to be separate on the substrate and doped to have the opposite polarity to the substrate; a channel region disposed between the source region and the drain region; an insulating layer having the sensing surface which is disposed on the channel region and composed of an electrically insulating material; and a reference electrode disposed above and separate from the insulating layer. The method includes providing a first sample having a first target bio-molecule to a sensing surface of the field effect transistor and measuring a change in electric signal of the field effect transistor.