METHOD OF DETECTING BIO-MOLECULES USING THE SAME FIELD EFFECT TRANSISTOR

摘要

A method for detecting bio-molecules is provided to detect the bio-molecules accurately and easily and prepare a field effect transistor(FET) simply and lower the distribution of performance among FETs significantly since the bio-molecules do not use a fixed FET. A method for detecting target bio-molecules such as nucleic acids and proteins using a field effect transistor comprises the steps of: (a) providing a first sample including a first target bio-molecule to a gate electrode of the field effect transistor and measuring the first electrical signal change of the transistor; (b) providing a second sample including a second sample to the gate electrode of the same field effect transistor and measuring the second electrical signal change of the transistor; and (c) comparing the first electrical signal and the second electrical signal by calculating the ratio of the first electrical signal to the second electrical signal, wherein the field effect transistor comprises a substrate(21) consisting of a semiconductor material, a source region(22a) and a drain region(22b), each of which is respectively formed in the substrate with being spaced apart from each other and doped with polarity opposite to that of the substrate, a channel region displaced between the source and drain regions, an insulation layer(23) placed on the channel region and consisting of an electrically insulation material and a gate electrode(24) displaced on top of the insulation layer with being spaced apart therefrom. The method is further comprises a step of supplying a solution not including a bio-molecule to the gate electrode of the transistor to wash it before the step (b).