Combination of avian cell markers

摘要

Combination of markers allowing to characterize avian cells of StX type, germinal cells or stem cells, comprises: at least a marker of a target gene, preferably expressed in the StX cells e.g. 1P06, 2contig58, 60S-L14, ATM, Bloom syndrome, BTEB4, CD9, and/or ZPC; at least a marker of target gene preferably expressed in the stem cells such as 1P06, 1P08-A09, activin RIIB, astacin, and/or wnt-11; and/or at least a marker of a target gene preferably expressed in the germinal cells such as TC97694, TGF RII, TGF RIII, tie2, tie-2, TR-alpha, vasa, VE-Cadherin, vera and/or Wisp-1. Combination of markers allowing to characterize avian cells of StX type, germinal cells or stem cells, comprises: at least a marker of a target gene, preferably expressed in the StX cells such as 1P06, 2contig58, 60S-L14, ATM, Bloom syndrome, BTEB4, CD9, CHD helicase, Clock, cwflo (FLJ10374), CXCR4, Dnmt2, enxl (Ho-peel 2), eomes, EWS, FGF-4, GATA-5, HOJ-1, N-AGN6P deacetylase, NCorl, NF2, p53, pml, rbm6, SA-2, SA-3, SARA, SCYE1, SEF, sf-1, SnoN, SOCS13, SSB-1, TC87479, T-cell APP 2C, TGF-beta2, WD40/FYVE-d protein 2, WD-RP3, Zan75 and/or ZPC; at least a marker of target gene preferably expressed in the stem cells such as 1P06, 1P08-A09, activin RIIB, astacin, Claudin-3, dapper-1, Dorfin, FPP synthase (fps), GalNAc-T3, gcnf, HSPb7, IRX4, LMX, pax-6, Slc38a2, sox-3, tral gp96, wnt-10a and/or wnt-11; and/or at least a marker of a target gene preferably expressed in the germinal cells such as adiponectin, BMP-2IK, Bruno like, CD34, CDK5 activator 1 dkk1, dkk3, DMRT1, emx2, endoglin, FAST-1, FGF R, FLJ00188, flk-1, gata-4, freezing, LHX9, NOS type III, plzf, PRL-R box11, PTEN, SAMSN-1, slug, smad3, Smarcd3, sox-9, Strat8, TACC2, TC95408, TC97694, TGF RII, TGF RIII, tie2, tie-2, TR-alpha, vasa, VE-Cadherin, vera and/or Wisp-1. Independent claims are included for: (1) a DNA chip, comprising the marker combination; (2) a characterization process of an avian cell comprising the analysis of gene expression expressed in the cell by the combination of markers and the characterization of the phenotype of the analyzed cells; and (3) culture process of avian cells comprising the cellular culture in a culture medium and their characterization.