发明名称 |
HOT START REVERSE TRANSCRIPTION BY PRIMER DESIGN |
摘要 |
The present teachings provide methods, compositions, and kits for performing primer extension reactions. In some embodiments, a reverse transcription reaction is performed on a target polynucleotide with a hot start primer comprising a blunt-ended self-complementary stem, and a loop, and extension products form at high temperatures but reduce extension product formation at low temperatures. |
申请公布号 |
WO2007011901(A3) |
申请公布日期 |
2007.10.04 |
申请号 |
WO2006US27753 |
申请日期 |
2006.07.17 |
申请人 |
APPLERA CORPORATION;LAO, KAI QIN;STRAUS, NEIL;LIVAK, KENNETH |
发明人 |
LAO, KAI QIN;STRAUS, NEIL;LIVAK, KENNETH |
分类号 |
C07H21/04 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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