发明名称 |
A STRAIN OF THE FUNGUS METARHIZIUM ANISOPLIAE FOR CONTROLLING SUBTERRANEAN TERMITES |
摘要 |
<p>Alates of the Formosan subterranean termite (FST)1 Coptotermes formosan?s Shiraki, collected after swarming in 2002 died within 48 hrs, and the cadavers were visibly infected with a fungus. Fungi were picked from the cadavers, transferred to media and ultimately isolated to purity. The individual fungal cultures were then used to infect FST workers. A single fungal isolate, C4-B, taxonomically identified as Metarhizium anisopliaß (Metschnikoff), was found to cause rapid mortality of FST alates. This is the first report of a biological control agent for termite alates. In initial experiments C4-B was more lethal to both alates and workers when compared to M. anisopliae strain ESC1, previously marketed as the termite biocontrol agent BioBlast®. Dose response assays in which FST alates were exposed to a known concentration of C4-B spores revealed that 106 spores/µl killed 100% of the alates in 3 days; both 105 and 104 spores/µl required 6 days, 103 spores/µl, 9 days, and 10° spores/µl, 12 days. Assays with workers demonstrated that 106 and 105 spores/µl killed 100% of the workers in 6 days. In an experiment to test the transfer of inoculum from infected workers to uninfected nestmates, 62.8% of the workers died in 21 days when only 20% of the workers had been inoculated. Mortality of alates caused by C4-B was tested at two field sites by dispersing fungal spores on grassy lawns and collecting alates from the treated areas. Alates thus infected showed 100% mortality by day 5, while only 64.8% untreated control alates from the same collection area were dead on that day.</p> |
申请公布号 |
WO2007112257(A2) |
申请公布日期 |
2007.10.04 |
申请号 |
WO2007US64614 |
申请日期 |
2007.03.22 |
申请人 |
THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF AGRICULTURE;RAINA, ASHOK, K.;WRIGHT, MAUREEN, S.;LAX, ROBERT, R. |
发明人 |
RAINA, ASHOK, K.;WRIGHT, MAUREEN, S.;LAX, ROBERT, R. |
分类号 |
A01N65/38;A01N63/04 |
主分类号 |
A01N65/38 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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